Representing Umbrella Corp at Biotech Industry Growth Discussion and Intellectual Contribution Kvell.

I was recently asked to share my research at Umbrella Corp. I may also get to do a speaking gig at the Biotech Industry Growth Discussion and Intellectual Contribution Kvell.

My work in nerve regeneration and weaponizing (being the best way to describe the planned process of repair) of certain DNA proteins has been an important goal for the biotechnology dept. of Umbrella corp.

Three mechanisms of repair to the double-strand breaks (DSBs) and flaying damage to axons is being worked on here at Umberlla Corp. Two have been partially published in certain journals so legally I have no trouble sharing some of this information with you.

Anon-homologous end joining (ANHEJ),

Using extracted Uracil-DNA glycosylase from nematodes we are designing DNA-binding proteins that have a specified DNA-binding site for regeneration, augmentation and enhancing of spinal nerve endings as well as the cognitive brain functions of reposing test subjects.

By placing single Zinc finger protein to the severed end of certain flat worms we have been able to, through said flat worm implantation, been able to create a "bridge" across failed nerve endings. This along with specific gene protein protocols our group has been able to bring positive response back from and into unresponsive (dead) muscular tissue. Using this same process we have been able to generate certain responsive activity in the cognitive brain areas of cadaverous test subjects.

Recently transcription activator-like effector nucleases (TALENs) have been created, which are based on natural proteins secreted by Xanthomonas bacteria via their type III secretion system when they infect various plant species. These along with specific growth gene proteins and gestation hormones from Neotomys ebriosus we have been able to create some control over the test subjects.

To see if the nematodes can "automatically" find and repair broken axons as they transverse through the host body the DOE is to plant many of these modified nematodes into a test subject. While "automatic" repair of mortified test subjects is evident, there is still massive a loss of higher reasoning and fine motor control.

When the test subjects gain full mobility they are single minded and focused on DNA extraction. It seems that for now the instinctive memories and survival of the "bridge" symbiotes is to obtain more DNA from subsisting sources for further repairs.

Micro-homology-Mediated End Joining (MMEJ),

Using Trans-lesion synthesis (TLS) as a DNA damage tolerance process we are allowing the DNA replication machinery to replace past DNA lesions with thymine dimers to the axons AP sites. This involves switching out regular DNA polymerases for specialized trans-lesion polymerases (i.e. DNA polymerase IV or V, from the Y Polymerase family)

The ultimate goal being is the use of bacterial cells and plasmids to replicate the same repair process of the nematode but in a smaller vehicle. Progress here has been slow but there is some positive direction, By controlling the formation of pyrimidine dimers with irradiation from high wavelength UV light, the results in an abnormal covalent bond between adjacent pyrimidine bases have shown to be receptive to photo-reactivation process directly reversing the damage to the broken cells by the action of the enzyme photolyase.

Another type of base excision repair (BER) to the severed and or reposed nerve cells, which repairs damage to a single DNA base pair caused by death is alkylation-hydrolysis which has shown great promise in re-animation. The damaged base and glycosylase is removed by the gestation androgynous hormone of a paddocstol and using phonosemantic matching from inert Ebola DNA. The "missing tooth" is then recognized by an enzyme called AP endonuclease, which cuts the phosphodiester bond. The missing part is then resynthesized by a DNA polymerase where TALENs take control of the growth, and the DNA ligase performs the final nick-sealing step.

Yes, the Nucleotide excision repair (NER), is clumsy and not totally controllable, but with the Zinc finger protein as a marker and extracted Uracil-DNA glycosylase in a synthetic reproduction environment the bacteria in specialized synthetic polymerase plasmid envelopes should recognize the bulky, helix-distorting lesions such as pyrimidine dimers and 6,4 photoproducts. With the specialized form of NER known as transcription-coupled repair, the new self-replicating organism will deploy NER enzymes to genes that are being actively transcribed.

Generation and control of self-replicating and repairing, methylation of guanine bases, is directly accelerated by the protein Methyl Guanine Methyl Transferase (MGMT), the bacterial equivalent of which is called OGT. Right now this is an expensive process because each MGMT molecule can be used only once. But we are also on the verge of being able to force the MGMT to replicate itself using some of the DNA from the inert Ebola virus DNA mentioned before.

The nerve cell and brain cell reaction is stoichiometric rather than catalytic. And a generalized response to the methylation agents in the bacteria are the adaptive response to and from the gestation androgynous hormone of a paddocstol and confers a level of acceptance to alkylating agents upon sustained exposure by upregulation of alkylation repair enzymes. The DNA damage is reversed by neighboring cells as the repaired/replaced DNA is certainly traded by methylation of the base pairs using the cytosine and adenine found in the delivering plasmid envelope.

Homologous Recombination.

This, I am told cannot be talked about without proper NDA filings and disclosure of asset investments to the proper SEC channels

 

As you can see we are very busy at Umbrella Corp and "We have your future in control."

Regards

John Sleestaxx

A New Baseball Story

I have not posted any baseball stories in a long while because they all are basically the same story; One team gets their asses handed to them and they bitch and complain the whole game until I either throw the coach out or I call ball game.

This weekend I scheduled 7 games. Seven games in one day, for those that know, 7 games is a VERY long day, more than 14 hours of baseball. Yeah, there is a 20 minute break in the schedule but rarely does a game end on time or start on time so there is no break except to turn in the score card, grab some baseballs and water head back out to the field.

 So Saturday in the 4th game the team that refused to practice and felt that the sport is played by complaining was doing their part to win. The coach asked why every ball call was not a strike. Seriously they wanted a pitch 4 balls off the plate and into the batter’s box called a strike. They wanted to know why a tie did not go to the runner.

"Com'on blue seriously that was a tie, and the tie always goes to the runner."

"No coach there is no “tie” in baseball. The word "tie" is not even in the rule book." "The rule reads 'the batter-runner must beat the ball to the base in order to be called safe'"

"Oh my god the catcher did not even move his glove."

"Yes you are right he did not and he is holding it 12 inches off the plate."

"Jesus Christ!" exclaiming as he is throwing his hat into the dirt. "I swear to god blue, you are doing this on purpose."

"Time!" I say holding up my hands. "Coach, please come here."

The coach walks over smirking to his peanut gallery bleachers, and gets 1 step too close to me, on purpose. I do not step back, I lean in 1 inch and say "Really? Who are you that I would care that much to do something like that to you?"

"I don’t know" he says "but you have not given us one call."

I smile "Given you a call? No one gives anybody a “call” in this game. In fact this game was already decided on Wednesday night with software."

"Wait what?" He steps back and blinks. "Already decided?"

"Yes sir" I hold my poker face. "Yeah it’s just like wrestling; baseball has been a scripted sport from the beginning."

"Bullshit!"

"Now hold on coach, keep it down, all sports are scripted even the Olympics. Well except high school. obviously." 

"Fuck you!" He says looking at me like I just said something about his mom.

"Now" Placing my hand on his shoulder and slightly guiding him towards his dug out. "Here’s what I am going to do, I am not going to throw you out for cussing or for behaving like a poor sport. I am telling a secret and if you do not like it, then you should go and talk to the tournament director. Tell him what I told you and tell him that I said I felt bad about you paying your tournament fees, and that he should reimburse you, because I said you were a nice guy and should not be taken advantage of like this." we ended our short walk at the gate and my partner had already opened the gate and he passed through the gate and we shut it.

The look on his face was priceless, it was a mixture of dumb ass and 'what there is no Santa?'

As we turned to get back to the game, he called after us, "Hey blue? Are you serious?"

I turned back and looked at him with the dead pan seriousness of a reporter, "Coach, is this your first game today or your second?"

He looked even more bewildered "Our first."

I made a face resembling pain, "oops you weren't supposed to lose the first game." Sucking air through my teeth and glancing at my partner who was now smiling and facing outfield, "It was supposed to be the second game; I got the whole thing screwed up. I am very sorry; really you should go talk to the tournament director. Tell him what happened, tell him I got your two games mixed up, He will know what to do. Tell him I told you he should make it right for you."

We finished the game with a run rule, 20 run difference , (23-3) after 3 innings of baseball 1 hour and 5 mins.  That gave us a break of about 25 minutes.

My partner and I went out to our cars to change and get a drink of water.

At the cars, the tournament director and the "Umpire In Charge" walk up. "Hey ahh John," The tournament director started, "what happened on your field just now?"

Squinting into the 3:00 sun "Why what do you mean?"

 The tournament director continued, "I had the craziest conversation with the East Nicolas coach. He said you told him that all the games were rigged and that he was not supposed to lose the first game but only loose the second and you made a mistake."

"Yeah" I said, "he was acting like a jerk and making the game personal, so I told him some stuff to get him off the field."

"Why would you tell him that?"

“I dunno it seemed kinda funny and it was either be funny or be a lunatic I choose funny. Did you keep him in the office?”

“Yeah, Thanks. I do not need your craziness in the office!”

“And I do not need their craziness on the field.”

“You know you have his team at the 8:00 game? “

“So?” I said, "he was ejected you have to bar him from the next game.

"well," the UIC was now smiling, "you did not eject him so I have to let be in the next game."

“Crap!” I exclaimed.

“Exactly!” They both said with big ass, stupid smiles on their faces.

Needless to say the 8:00 game was with no events, no complaining, I do not know what the coach was thinking as he watched his team win the next game. Did he think they actually won? I have no idea. The game ended 3-2 in favor of East Nicolas and I went home.

You bunch of whining pussies

I placed the song lyrics online because I was giving tribute to the great bands of my time and because the songs fit the theme of this Blog.
You pussies can stop whining.
I know the history of this week but you all need to lighten up.
I spend too much time being considerate of everyone else but there is not one motherfucker that is concerned about me.
If they were then 50% of my opportunities would not be taken away. I would be afforded the same opportunities as everyone else regardless of my gender or race.
So fuck off if the lyrics are offensive today.
Maybe today time has to change and we need to stop pretending to care about everybody else and face reality that the ones that have the power to make a change choose not to while the rest of us struggle to make a change against a tide that is just too fucking big.
You do not like what I have to say then go the fuck away. If you do agree then say so and let everyone else know how you feel because I am sure that we out number the pussies.